FastQCFastQC Report
Wed 3 Mar 2021
TC3_A6_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_A6_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56941437
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC2501960.4393917912538807No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2466510.43316609659851046No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1745130.30647803988508404No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1403880.24654804549453152No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1296280.22765143773944446No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1219120.21410067329351032No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA1135010.19932935658086748No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT1031580.18116508018580565No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1010840.1775227414791095No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC806730.1416771410247339No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA730100.12821945466532572No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC717720.12604529105930362No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA660380.11597529581137897No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAA579350.10174488571477393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)