FastQCFastQC Report
Wed 3 Mar 2021
TC3_B2_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_B2_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50025476
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2316690.46310204024845264No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC1873770.3745631525824962No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1788650.35754782223361553No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1367470.27335472030291125No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1313310.262528236612881No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1302740.26041531318962363No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1286670.25720294995293996No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT982540.19640792623342557No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA902400.18038808866106543No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA643720.12867843576340984No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC607000.12133817577268032No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA600640.12006682355206375No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC526570.10526036773743043No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAA526160.1051784094967932No Hit
GATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCT525470.10504047977474516No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC518050.1035572355173592No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG516100.1031674341289626No Hit
CAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATC516090.10316543514748365No Hit
GACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGTATGATGGATTCAA504860.10092057894661513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)