FastQCFastQC Report
Wed 3 Mar 2021
TC3_B4_mPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_B4_mPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44781343
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1109180.24768797130537154No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1067500.23838052378196878No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1022640.22836295910107027No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT884960.19761801248345767No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG825620.1843669583558492No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC790950.17662489488088823No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT769660.17187068284218274No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT710580.15867768860795445No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC702990.15698278633581847No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA702630.1569023957142152No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA687500.1535237565340548No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT665000.14849934268384937No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT640680.14306850957998288No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC633720.14151429089565268No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG585600.13076874447468No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT571500.12762011179521793No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC571070.12752408966385845No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT548550.12249520966800839No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA546220.12197490370040934No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT535520.11958551578053386No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA532790.11897588690004228No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG530480.11846004707808785No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG522670.11671601720386099No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC515690.11515733237388616No Hit
CTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGC501960.11209132338884968No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG495940.11074701354981695No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA487140.10878190946618103No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA462160.10320369355604185No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT453570.10128548400167453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)