FastQCFastQC Report
Wed 3 Mar 2021
TC3_B5_mPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_B5_mPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47647323
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC1129000.23694930353170104No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1002830.21046932689167025No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT840240.1763456889277914No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT810380.170078810093906No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA767950.16117379773885723No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC699790.14686869186753682No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT662790.13910330282353953No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG655890.13765516270452383No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT653170.13708430167210023No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT650510.1365260331624507No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC614260.12891805065312903No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC612750.12860113882998211No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA581010.12193969428250986No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG550980.115637136634098No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT540240.1133830750575431No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC533680.11200629256758034No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT513560.10778359993068236No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG501750.10530497169799025No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG501560.10526509537587243No Hit
CACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT486780.10216313726586486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)