FastQCFastQC Report
Wed 3 Mar 2021
TC3_B6_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_B6_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55038337
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC1987320.36107922374180745No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1862820.3384586274836029No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1077450.19576354568997967No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1043990.1896841468883771No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA962680.17491080807910311No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT948760.17238166189505325No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC902330.16394572386880074No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC897420.1630536184260073No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA884870.16077338964656582No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT650300.11815400599767395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)