FastQCFastQC Report
Wed 3 Mar 2021
TC3_D1_fPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_D1_fPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54307191
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG1201370.2212174811251055No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG908310.16725409347723397No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG785740.1446843383963645No Hit
CTCTGATCTTCAGGAAGTGTACAACACTTGGTGCCCACTCTTCCTAGGTT695870.12813588535632417No Hit
GTTGGGTTTTCCTTAAAGGAGGTGCACATGGCCTCAGCCTCTGGCCTTTC672840.12389519465295122No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT658340.12122519833515234No Hit
GTCCTCAACAAAATCAGCAGCAATGGCAGGCAGATCAGCAGGCATGGTGT641400.11810590608525491No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG592870.10916970461609772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)