FastQCFastQC Report
Wed 3 Mar 2021
TC3_D5_fPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_D5_fPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44128373
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG989740.22428653782454203No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG611710.13862056505006426No Hit
GTTGGGTTTTCCTTAAAGGAGGTGCACATGGCCTCAGCCTCTGGCCTTTC605210.13714758982843078No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG539340.12222068554396963No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT532630.12070012189209875No Hit
CTCTGATCTTCAGGAAGTGTACAACACTTGGTGCCCACTCTTCCTAGGTT491080.11128441105227242No Hit
GTCCTCAACAAAATCAGCAGCAATGGCAGGCAGATCAGCAGGCATGGTGT487860.11055472178863246No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG457950.10377676965339284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)