FastQCFastQC Report
Wed 3 Mar 2021
TC3_D6_fPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC3_D6_fPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47751478
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG1165200.24401338949131585No Hit
GTTGGGTTTTCCTTAAAGGAGGTGCACATGGCCTCAGCCTCTGGCCTTTC829360.17368258213913296No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG666980.1396773519764142No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTG637900.13358748811921592No Hit
GTCCTCAACAAAATCAGCAGCAATGGCAGGCAGATCAGCAGGCATGGTGT634900.13295923531414042No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT634760.13292991684990357No Hit
CTCTGATCTTCAGGAAGTGTACAACACTTGGTGCCCACTCTTCCTAGGTT606120.12693219673744968No Hit
CGGGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTC519070.10870239451017621No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG518080.1084950710845013No Hit
CTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCATAGTTTTCACGGAGGT489720.10255598790052112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)