FastQCFastQC Report
Sun 7 Feb 2021
TC6_A1_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A1_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71103561
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC4504880.6335660178820017No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC3894000.5476518960843607No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT3857150.5424693145818674No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT2508620.3528121467784152No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA2271500.31946360604921037No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC2267560.3189094847162437No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT2056770.28926399340252457No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG1105430.15546760027954157No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC942690.13257985770923625No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA833180.11717837873127057No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA778180.1094431824588926No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA761640.1071169979798902No Hit
AGAAGATAATGGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGG718940.1011116728738804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)