FastQCFastQC Report
Sun 7 Feb 2021
TC6_A1_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A1_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71103561
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1485040.20885592495149433No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT1449480.20385476896157143No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1432160.20141888533543348No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1330670.18714533861391272No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1294850.1821076162416113No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC1135370.1596783598503597No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG1081280.1520711459163065No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA925270.1301299100898758No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT869370.12226813787849528No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG868300.12211765315101447No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC859280.1208490809623445No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC833080.11716431473804806No Hit
CCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAAT771760.10854027409400777No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA768850.10813101189123284No Hit
CGTAGAACTAGCTTCTTCTGCATGGACACAGACTAGGGTCAGTCCCAAAC722780.10165173021362461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)