FastQCFastQC Report
Sun 7 Feb 2021
TC6_A2_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A2_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74061858
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC3099890.4185541766991587No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC3048240.4115802765844735No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2362840.3190360144623971No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1838930.2482964983136124No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1676180.22632162428331193No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1659570.22407890442068035No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1479920.1998221540701828No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC1151500.1554781409885774No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA891420.12036154966568623No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA885600.11957572006902663No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA859000.11598412775439687No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC842930.11381431991619763No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT833450.11253430882060776No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG831880.11232232386068414No Hit
AGAAGATAATGGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGG746490.10079277244165277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)