FastQCFastQC Report
Sun 7 Feb 2021
TC6_A3_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A3_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67792403
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1757430.25923701214721656No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT1432400.21129211189047245No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1346900.19868007924132738No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT1297760.19143147942402927No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1170080.17259751066797263No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA1101940.16254623692864228No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTATG929330.13708468189274836No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC908610.1340282922261953No Hit
CTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAATT883590.13033761319834022No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA841640.12414960419680064No Hit
CCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTCTG817270.12055480611890981No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA773520.11410128064054612No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT731720.10793539801207519No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC720150.10622871710271135No Hit
CCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTC711250.10491588563396993No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC697670.10291271132548584No Hit
CTCCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTG690210.1018122930382037No Hit
CCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAAT685600.10113227583922642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)