FastQCFastQC Report
Sun 7 Feb 2021
TC6_A5_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A5_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62513463
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1593320.25487629760648517No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC1518920.24297486127108334No Hit
CTGAGAAGTCCAATTCCAGTCTATCCACATGTTACCTAGGATACCTCATC1153630.18454104838185015No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1140670.18246789495568339No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC1130650.18086504022341554No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1108170.1772690148360522No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC878500.14052972877218464No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA835490.1336496108046358No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC831220.1329665579396873No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA829570.13270261479515222No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA788630.12615362549983833No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT788270.12609603790466703No Hit
ATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAATTCCAGT788080.12606564445165996No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA756300.12098193952237135No Hit
CCACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTG732180.1171235706458943No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT688220.1100914854133101No Hit
CAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATC676660.10824228374614281No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCT673070.10766800744985124No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC642520.10278106013739792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)