FastQCFastQC Report
Sun 7 Feb 2021
TC6_A5_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A5_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62513463
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1622310.25951369867319624No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA1057080.16909637528799196No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA973380.15570725941066488No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA921330.14738105294214784No Hit
GGTGTATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATC888640.14215177936950957No Hit
CTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAATT837850.1340271294840921No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC827340.13234589163617444No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT796320.12738376051891415No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATGTTCGTGTAGATCT767660.12279914808111016Illumina Single End PCR Primer 1 (97% over 36bp)
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC659120.10543648813696338No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA658130.10527812225024231No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTTTC655720.1048926052936789No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA654330.10467025319010083No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT637160.10192364483151413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)