FastQCFastQC Report
Sun 7 Feb 2021
TC6_A6_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_A6_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62066654
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC2624670.42287924849307973No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC2356560.3796821397847546No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1787430.287985558235506No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1566870.2524495681690848No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1327480.21387974289704742No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1292100.20817941949955932No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1176560.1895639484609562No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC1140740.18379273353449985No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC945510.1523378398970887No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA929580.14977124431421743No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA878070.14147210191159976No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA866430.13959669873616837No Hit
CTGAGAAGTCCAATTCCAGTCTATCCACATGTTACCTAGGATACCTCATC859670.13850754706383883No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC858090.13825298202799846No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT752700.1212728496690026No Hit
CCACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTG746890.12033675925239985No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCT736140.11860475030601779No Hit
CAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATC735040.11842752148359731No Hit
ATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAATTCCAGT696520.11222129035665432No Hit
AGAAGATAATGGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGG684400.11026855096780311No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG665660.10724921630220312No Hit
CCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTAT644180.10378842075166482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)