FastQCFastQC Report
Sun 7 Feb 2021
TC6_B2_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B2_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71362067
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTTACGGGTGTAGATCT1682190.23572607559139225Illumina Single End PCR Primer 1 (96% over 32bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1431520.20059957063743683No Hit
CTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAATT853610.11961677062969603No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA795500.11147378900894225No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA789720.1106638348914417No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA751440.10529964049387751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)