FastQCFastQC Report
Sun 7 Feb 2021
TC6_B3_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B3_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68839766
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGGCGTAGTGTAGATCT936320.13601440771893383Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT884840.1285361719561917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)