FastQCFastQC Report
Sun 7 Feb 2021
TC6_B5_mPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B5_mPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68145889
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC2407280.3532538844712995No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC2013080.2954074016115631No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1626230.23863948711564975No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1325820.19455612355427632No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1165840.17108001922170243No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1138890.1671252685543511No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1007220.14780348672243457No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC822610.12071307779109022No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC796310.11685371072053957No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA706870.1037289278007658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)