FastQCFastQC Report
Sun 7 Feb 2021
TC6_B5_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B5_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68145889
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1311540.1924606193045629No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1047340.1536908558049628No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT972530.14271293753317973No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA882980.12957201277394736No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA765460.11232665847238416No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT758040.1112378180289056No Hit
CTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAATT714800.10489260768173411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)