FastQCFastQC Report
Sun 7 Feb 2021
TC6_C4_fCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_C4_fCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66202574
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAAGCAGTGTAGATCT1773410.26787629133574176Illumina Single End PCR Primer 1 (97% over 34bp)
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTC1063970.1607142948852714No Hit
ATTTTTTATTAATTGCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAA1011790.1528324261228876No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTC781040.11797728589827944No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG689990.104224044219187No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT663250.10018492634440467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)