FastQCFastQC Report
Sun 7 Feb 2021
TC6_D6_fPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_D6_fPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62214976
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTAGATCT1223190.19660700343274262Illumina Single End PCR Primer 1 (96% over 32bp)
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTC894870.1438351434869958No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG763970.12279519323450352No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTC664900.1068713745063568No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT640530.10295431119349784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)