FastQCFastQC Report
Tue 4 May 2021
TC6_B1_mPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B1_mPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68706033
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC2162980.3148166042420176No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC2082500.30310293129571314No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1517390.22085251232595543No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT1327270.1931809976570762No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1188010.17291203525023777No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA1114110.16215606568348956No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1069900.15572140513483002No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC1002340.1458882075173806No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC910970.13258952092314805No Hit
TGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGA817720.11901720479189942No Hit
CTGAGAAGTCCAATTCCAGTCTATCCACATGTTACCTAGGATACCTCATC793780.11553279462372684No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA772780.11247629447620705No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT771550.11229727089613803No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC756100.11004856007331991No Hit
CCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCACAACTATGTGAGGA737750.10737776113489189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)