FastQCFastQC Report
Tue 4 May 2021
TC6_B1_mPM_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B1_mPM_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68706033
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT1534190.22329771244397126No Hit
GCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAA1060630.15437217864113914No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA1012320.14734077282558286No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT931670.13560235678284613No Hit
CTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAATT855560.12452472696247795No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA842810.12266899473005523No Hit
CTCCAGAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGT793400.11547748652581935No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT742120.10801380426082816No Hit
GGTGTATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATC717250.10439403480040829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)