FastQCFastQC Report
Tue 4 May 2021
TC6_B2_mPM_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC6_B2_mPM_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71362067
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC1357650.19024813280702757No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC1345230.18850771236769248No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGAC977940.13703919198416717No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT974690.13658376795616078No Hit
CTGAGAAGTCCAATTCCAGTCTATCCACATGTTACCTAGGATACCTCATC966250.1354010667880458No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT928890.13016579242302498No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC777700.10897946664016893No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC763450.10698260744044871No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA730500.10236530844881497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)