FastQCFastQC Report
Thu 19 Nov 2020
TCDD_M_A11_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTCDD_M_A11_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36328547
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG731570.2013760693484383TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATG437670.12047550374090106TruSeq Adapter, Index 2 (97% over 37bp)
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC383980.10569649262328054No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATA383820.10565245012414068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)