FastQCFastQC Report
Thu 19 Nov 2020
TCDD_M_A12_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTCDD_M_A12_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38512748
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC530430.13772842176829345No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATA522900.13577322501110542No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTC458200.11897359284775005No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG401930.10436284629702353TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)