FastQCFastQC Report
Tue 26 Jan 2021
G124-G125-M20M38-NEBNext1_ATCACG-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext1_ATCACG-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619348
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1215070.8311382970020277No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1114580.7624006214230621TruSeq Adapter, Index 1 (100% over 50bp)
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC605790.41437552481820666No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA483860.33097235252899104No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG335890.22975716837713966No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG295460.20210203628780163No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC237280.16230545986045342No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG225210.1540492777106065No Hit
GTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAG202930.13880919997252955No Hit
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG200940.13744799015660616No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA200300.13701021413540468No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC192900.13194842889026243No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC185360.12679088014048231No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA174580.11941709028336968No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT172750.11816532447274665No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG171320.11718716867537458No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT170010.11629109588197778No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC157350.10763133896258574No Hit
AGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGG150530.10296628823665735No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG149350.10215913869756707No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA146530.10023018810414802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)