FastQCFastQC Report
Tue 26 Jan 2021
G124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16529069
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5487213.319733252973897TruSeq Adapter, Index 4 (100% over 50bp)
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA1653521.0003709222824346No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC544640.32950434171458776No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA491250.29720367190674807No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG371230.22459220177494568No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG275350.16658530495577215No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC241660.146203031761801No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT205980.12461681901140349No Hit
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC193600.11712698398197745No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT181880.11003644548885361No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC173110.10473064151405019No Hit
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG169900.10278860836021678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)