/***************************************************************************** maskFastaFromBedMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "maskFastaFromBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "maskFastaFromBed" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void ShowHelp(void); int main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string fastaInFile; string bedFile; // output files string fastaOutFile; // checks for existence of parameters bool haveFastaIn = false; bool haveBed = false; bool haveFastaOut = false; bool softMask = false; // check to see if we should print out some help if(argc <= 1) showHelp = true; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) ShowHelp(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-fi", 3, parameterLength)) { if ((i+1) < argc) { haveFastaIn = true; fastaInFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-fo", 3, parameterLength)) { if ((i+1) < argc) { haveFastaOut = true; fastaOutFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-bed", 4, parameterLength)) { if ((i+1) < argc) { haveBed = true; bedFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-soft", 5, parameterLength)) { softMask = true; } else { cerr << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } if (!haveFastaIn || !haveFastaOut || !haveBed) { showHelp = true; } if (!showHelp) { MaskFastaFromBed *maskFasta = new MaskFastaFromBed(fastaInFile, bedFile, fastaOutFile, softMask); delete maskFasta; return 0; } else { ShowHelp(); } } void ShowHelp(void) { cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; cerr << "Summary: Mask a fasta file based on feature coordinates." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -fi -out -bed " << endl << endl; cerr << "Options:" << endl; cerr << "\t-fi\tInput FASTA file" << endl; cerr << "\t-bed\tBED/GFF/VCF file of ranges to mask in -fi" << endl; cerr << "\t-fo\tOutput FASTA file" << endl; cerr << "\t-soft\tEnforce \"soft\" masking. That is, instead of masking with Ns," << endl; cerr << "\t\tmask with lower-case bases." << endl; // end the program here exit(1); }