/***************************************************************************** subtractMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "subtractBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "subtractBed" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void ShowHelp(void); int main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedAFile; string bedBFile; // input arguments float overlapFraction = 1E-9; bool haveBedA = false; bool haveBedB = false; bool haveFraction = false; bool forceStrand = false; // check to see if we should print out some help if(argc <= 1) showHelp = true; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) ShowHelp(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-a", 2, parameterLength)) { if ((i+1) < argc) { haveBedA = true; bedAFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-b", 2, parameterLength)) { if ((i+1) < argc) { haveBedB = true; bedBFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-f", 2, parameterLength)) { if ((i+1) < argc) { haveFraction = true; overlapFraction = atof(argv[i + 1]); i++; } } else if (PARAMETER_CHECK("-s", 2, parameterLength)) { forceStrand = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBedA || !haveBedB) { cerr << endl << "*****" << endl << "*****ERROR: Need -a and -b files. " << endl << "*****" << endl; showHelp = true; } if (!showHelp) { BedSubtract *bs = new BedSubtract(bedAFile, bedBFile, overlapFraction, forceStrand); delete bs; return 0; } else { ShowHelp(); } } void ShowHelp(void) { cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; cerr << "Summary: Removes the portion(s) of an interval that is overlapped" << endl; cerr << "\t by another feature(s)." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -a -b " << endl << endl; cerr << "Options: " << endl; cerr << "\t-f\t" << "Minimum overlap required as a fraction of A." << endl; cerr << "\t\t- Default is 1E-9 (i.e., 1bp)." << endl; cerr << "\t\t- (FLOAT) (e.g. 0.50)" << endl << endl; cerr << "\t-s\t" << "Force strandedness. That is, only report hits in B that" << endl; cerr << "\t\toverlap A on the same strand." << endl; cerr << "\t\t- By default, overlaps are reported without respect to strand." << endl << endl; // end the program here exit(1); }