FastQCFastQC Report
Wed 23 Nov 2016
G124-G125-M20M38-NEBNext1_ATCACG-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext1_ATCACG-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619348
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC842130.5760380011475205No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC470170.32160804982547786No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC468410.32040416576717373No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC383860.2625698492162578No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC361490.24726820922519938No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC252570.17276420261697034No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196270.13425359325190153No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC175450.12001219206219046No Hit
GGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCCATCGCCTC170210.11642790088860323No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG167680.1146973175547911No Hit
CGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCCATCGCCT166890.11415693777862049No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT161240.11029219634145107No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC147700.10103049739290698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG138700.042.26192510
CGTCGTG139350.042.08145512
TCGTGTA139550.041.7568814
CGTGTAG139950.041.74287815
GATCGGA144500.041.065641
GAGCGTC142800.041.032699
AGAGCGT144450.040.7320258
GATCTCG106700.040.48273534
AAGAGCG145250.040.449847
ATCTCGG109750.039.7186935
ATCGGAA150150.039.2469372
GTCGTGT150750.038.8946413
CGGAAGA151750.038.7124144
TGTAGGG153200.038.42921417
GTAGGGA153400.038.15561318
TCGGAAG155700.038.1351853
GTGTAGG155400.038.04377416
GTAGATC118450.037.7103231
TGGTCGC100850.036.88171442
TAGGGAA158700.036.7800519
Produced by FastQC (version 0.11.2)