G124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16489896
Sequences flagged as poor quality	0
Sequence length	50
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	558734	3.3883415638279346	Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC	103912	0.6301555813329569	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	50139	0.30405892189981065	No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC	45554	0.2762540163988906	No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC	45358	0.275065409751523	No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC	29963	0.18170520905650345	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	24883	0.1508984653390173	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG	22985	0.13938838668236597	Illumina Single End PCR Primer 1 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	22955	0.1392064570934832	No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC	19533	0.11845435532158602	No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC	18377	0.1114440018299691	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCCG	18074	0.10960651298225288	Illumina Single End PCR Primer 1 (98% over 50bp)
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT	16532	0.10025533211367736	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	105550	43.584988	10
GATCGGA	106660	43.572758	1
CGTCGTG	105895	43.56754	12
TCGTGTA	104980	43.563587	14
CGTGTAG	105800	43.464684	15
GAGCGTC	106055	43.44762	9
AGAGCGT	106655	43.372574	8
AAGAGCG	106175	43.316673	7
ATCGGAA	106965	43.224266	2
GATCTCG	78335	43.20025	34
GTCGTGT	107040	43.103603	13
ATCTCGG	79285	43.088	35
TCGGAAG	107700	43.080257	3
CGGAAGA	106635	43.070335	4
GTAGGGA	106850	42.95122	18
TGTAGGG	107990	42.89392	17
TAGGGAA	106855	42.827198	19
GTAGATC	82440	42.77175	31
GTCGCCG	68905	42.753143	44
GAGTGTA	97860	42.65858	27