Basic Statistics
Measure | Value |
---|---|
Filename | G124-G125-M20M38-NEBNext4_TGACCA-_AC8KUAANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16529069 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 548721 | 3.319733252973897 | TruSeq Adapter, Index 4 (100% over 50bp) |
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA | 165352 | 1.0003709222824346 | No Hit |
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC | 54464 | 0.32950434171458776 | No Hit |
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA | 49125 | 0.29720367190674807 | No Hit |
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG | 37123 | 0.22459220177494568 | No Hit |
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG | 27535 | 0.16658530495577215 | No Hit |
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC | 24166 | 0.146203031761801 | No Hit |
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT | 20598 | 0.12461681901140349 | No Hit |
GGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCC | 19360 | 0.11712698398197745 | No Hit |
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT | 18188 | 0.11003644548885361 | No Hit |
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC | 17311 | 0.10473064151405019 | No Hit |
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG | 16990 | 0.10278860836021678 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 67090 | 0.0 | 43.72604 | 1 |
ATCGGAA | 67500 | 0.0 | 43.3865 | 2 |
CGTCTGA | 67870 | 0.0 | 43.021603 | 16 |
ACGTCTG | 67820 | 0.0 | 43.017612 | 15 |
TCGGAAG | 68210 | 0.0 | 42.925213 | 3 |
CGGAAGA | 68595 | 0.0 | 42.642612 | 4 |
AATCTCG | 61015 | 0.0 | 42.391155 | 39 |
CTCGTAT | 64825 | 0.0 | 42.252506 | 42 |
ATCTCGT | 61475 | 0.0 | 42.227795 | 40 |
GCACACG | 69395 | 0.0 | 42.130802 | 11 |
ACACGTC | 69670 | 0.0 | 41.9891 | 13 |
TCGTATG | 67615 | 0.0 | 41.572292 | 43 |
CACGTCT | 70365 | 0.0 | 41.57124 | 14 |
CACACGT | 70430 | 0.0 | 41.545013 | 12 |
TCTCGTA | 62930 | 0.0 | 41.45366 | 41 |
ACTGACC | 67120 | 0.0 | 41.402996 | 32 |
ACCAATC | 62105 | 0.0 | 41.307175 | 36 |
GACCAAT | 63450 | 0.0 | 41.27734 | 35 |
GAACTCC | 70625 | 0.0 | 41.020584 | 21 |
CCAATCT | 62995 | 0.0 | 40.74453 | 37 |