G126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005

Basic Statistics

Measure	Value
Filename	G126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005_001.R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8449621
Sequences flagged as poor quality	0
Sequence length	50
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	835216	9.884656365060634	No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	183937	2.176866867756554	No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT	179718	2.1269356341544787	No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT	150754	1.784151028785788	No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT	102724	1.2157231667550534	No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC	74581	0.8826549735189306	No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	72640	0.8596835290008865	No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC	43102	0.5101057195346395	No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC	42896	0.5076677403637394	No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG	33269	0.39373363610036477	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT	32689	0.3868694229007431	No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT	24847	0.2940605265017212	No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT	22478	0.2660237660363701	No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	22156	0.26221294422554575	No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	18224	0.21567831267224885	No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT	17077	0.20210373932747988	No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT	15768	0.18661192022695455	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC	14772	0.17482440928415605	No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC	14200	0.16805487488728785	No Hit
CGTCGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	14068	0.1664926746418567	No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	13091	0.15493002585559754	No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA	12898	0.15264589973917173	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT	12728	0.15063397518066196	No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC	11934	0.14123710400738684	No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTC	11375	0.13462142266499288	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG	10722	0.12689326539024648	No Hit
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	10425	0.12337831483802647	No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	9979	0.11809997158452432	No Hit
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCT	9801	0.11599336822326115	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	9615	0.11379208605924455	No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCCAGATCGGAAGAGCACACGTCT	9532	0.11280979348067802	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT	9413	0.11140144628972116	No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG	9208	0.10897530196916523	No Hit
GTCGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT	8762	0.1036969587156631	No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA	8721	0.10321172985155193	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTTGGGG	45095	43.85204	1
CGTGGGG	143900	43.43702	1
GTTGGGG	26890	42.81674	1
CGTGTGG	14485	42.688736	1
ACCGCCT	5280	42.47485	1
GCCGTGA	7615	42.38101	1
TTGGGGG	74930	42.257492	2
CTAAACC	3880	41.93281	1
GTGTGGG	15060	41.536125	2
ACTTGCG	5065	40.981564	25
ATTCGTA	4380	40.858215	8
TTGTGGG	9670	40.708504	2
CGACACT	5440	40.697712	10
TAAACCA	4060	40.602356	2
CGGGTGG	5020	40.601326	1
ACGCACT	5600	40.537773	21
ACCCCAT	3840	40.530293	6
AACCATT	4440	40.497913	4
GATCATC	5500	40.446423	4
CTTGTGG	7420	40.409424	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content