FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7906814
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG81318010.284546974293313No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1787842.2611383042525093No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1249081.5797513385290205No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT986841.2480880415297488No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG720790.9116061159399981No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT712970.9017159123763376No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC616790.7800739969347957No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC358820.45381110520621837No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG341040.4313241717839828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA328380.41531266575892645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT292430.3698455534681858No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG259640.3283749940241417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA242350.3065077792395268No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT230310.2912804070008476No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT212030.26816110762185624No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC207230.2620903944370008No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG200230.2532372710424199No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG194620.24614212500762003No Hit
GAAGCAGTAGCAGAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCCCA184030.2327486140435326TruSeq Adapter, Index 1 (97% over 34bp)
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG180620.22843587821845818No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG147880.18702855537008964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA145990.1846382120535528Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC140910.17821337393291406No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC137360.17372357563994803No Hit
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG134520.17013173700557518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG133760.16917054075130641No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT131070.1657684119039603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA130240.16471868441574572No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC123440.1561185074038671No Hit
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG120810.15279226247133168No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC120360.15222313311025149No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT118650.1500604415381467No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCCAGATCGGAAGAGCACACGTCT112660.14248469737621247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC110540.13980346571956798No Hit
NTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG105270.13313832853536203No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC104240.1318356546644451No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG98680.12480374522532084No Hit
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG97190.1229192946741886No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG89190.11280143936609613No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG88510.11194142166490825No Hit
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCT88340.1117264172396113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT88310.11168847528220595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT88240.11159994404826015No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTGA84020.10626277537324136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTC80250.10149473605930276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGG406150.043.6167561
CGTGGGG1271150.043.3577161
GCCGTGA110150.043.0819051
CGTGTGG123800.042.963891
GTTGGGG185300.042.690781
TTGGGGG618250.042.0656932
GTGTGGG128550.041.672942
GAGTGAT53700.041.66872813
ACCGCCT48200.041.502761
CAGTAGC50350.041.2190675
AGTGATC53600.041.17465614
TTGTGGG77900.041.1707272
CAGTCCC37350.040.9642843
AAGCAGT43400.040.9253432
CTAAACC37250.040.8738751
CTCGCCC134600.040.8376351
ATTCGTG35750.040.59325811
TCGCCCG134600.040.502082
ACGCACG144800.040.46657618
ACCGGGT52050.040.45619614
Produced by FastQC (version 0.11.2)