FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7906814
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA100463512.705939459306872No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC1796692.2723311816870866No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC1127861.4264405359731491No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT510070.645101807124842No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT480650.6078933942293318No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAG347760.4398231702427805No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAA280870.35522525254799214No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGTACTGTAGAA259880.32867852968338446No Hit
GAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTG248620.3144376483372443Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC230820.2919254202767385No Hit
CGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCT228310.2887509431738245Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA219080.2770774676121128No Hit
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT208830.2641139654986193Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC185070.2340639352335846No Hit
GCAGAGTACTAACCACTATACGATCACGGATCGTCGGACTGTAGAACTCT175990.22258016945889963Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA155600.1967922857423989No Hit
TGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAGA155320.19643816080661566No Hit
GGCCTCGATCCGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA143260.18118549392966624No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAACGATCGTCGGACTGTAGA116540.14739185720063733No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAAC116490.14732862060496177No Hit
AAATGTATTGAATCCATCATACGTCACAGAGATCGTCGGACTGTAGAACT116090.14682272783955713No Hit
CGTCACAGAATATTCACCAGCCTTTTCTGTGATCGTCGGACTGTAGAACT115030.14548211201123487No Hit
GTCTGTCAGGACCACGTCTTTCTTGTGATGATCGTCGGACTGTAGAACTC106780.1350480737247645Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTG104440.13208860104714745Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGTCTGTCAGGACCACGTCTTTCTTGTGATGATCGTCGGACTGTAGAACT102250.12931883815655712No Hit
AATGTATTGAATCCATCATACGTCACAGAGATCGTCGGACTGTAGAACTC102140.12917971764607084Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTATAA100960.1276873339881272No Hit
GGGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTC96180.12164191544154193Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCTCGATAAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA95260.1204783620811113No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGGATCGTCGGACTGTAGAACTC94770.11985864344349065Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA92690.11722800106338659No Hit
ATGAGAGTAGTGGTATTTCACCGGCGGCGATCGTCGGACTGTAGAACTCT91410.1156091442140918Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCAGAGTACTAACCACTATACGATCACGGGATCGTCGGACTGTAGAACTC90020.11385116685431072Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAACT87870.11113199324026087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG2371050.043.5586931
AAGCCTA39100.041.5019651
GTGCGAT146000.041.4722631
TGCCTCG56450.041.38371
TTAAAAT35650.040.326983
CTTAGTA21250.040.2742088
AACCTTA14900.040.0484545
TAAAATC35700.040.0316934
CGATAAG55450.039.9425476
CTCGATA58900.039.741614
TCGATAA57750.039.6918565
ATGAGAG41100.039.3216861
GGCATCG51250.039.18131
AGAGTAC160900.038.860143
TTAGTAT22350.038.7864349
GGCCTCT59900.038.559071
CCTTAGT18200.038.2256627
GAGTACT161300.038.129764
TTTTAAA32850.038.0036741
AAGAAGG58650.037.889610
Produced by FastQC (version 0.11.2)