FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7059706
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG5470657.749118731006645No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1427942.022662133522274No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1219281.727097417371205No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT917571.2997283456279909No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT841151.1914802117821903No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA788571.1170011895679508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT526400.7456401158915116No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG470370.6662742046198524No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC360820.5110977709270046No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA342420.4850343626207664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC317360.4495371336993354No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG293480.4157113624845001No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG280830.3977927692739613No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG265110.3755255530471099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG238170.33736532371178063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAA236230.3346173339229707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT235850.33407906788186364No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC201660.28564928907804377Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC186380.2640053282672111No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT184690.26161146087386644No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT183180.25947256160525667No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG178150.25234761900849695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG172110.24379202193405788No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG144240.2043144572875981No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT143950.20390367530885847No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA131320.18601341188995688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC119500.16927050503236254Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG118740.16819397295014835No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT107310.15200349702947968No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG100500.14235720297700782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTGA96510.13670540954538332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT94830.13432570704785723No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCAGATCGGAAGAGCACACGTCTGA94450.13378744100675014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG87190.12350372664244093No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCTG86920.1231212744553385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT84880.12023163570834253No Hit
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG83890.11882931102230036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCGTCGCCGCGCTCTCTCTCTCCCCCAGATCGGAAGAGCACACGTCT76680.10861642113708418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC76140.10785151676287937No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT75500.10694496343048848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGG279150.043.74831
CGTGGGG941400.043.436131
CTAAACC80900.043.1028021
GTTGGGG170300.043.026441
ATTCGTA109900.042.177388
TTGGGGG468600.042.070432
ACCATTC111500.042.0185855
GCCGTGA68200.041.9396931
CTCGCCC228300.041.9081341
TCGCCCG227450.041.791062
ACGCACG252550.041.7183818
AACCATT92900.041.6747934
TAGACGA111250.041.59325813
GACGACC108350.041.5728315
CGTGTGG89750.041.5269171
ACGTTCA145700.041.519822
AAACCAT88100.041.498343
CGTCACG252800.041.484567
TTGAACG255000.041.43836614
GTCACGT250650.041.3989148
Produced by FastQC (version 0.11.2)