FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7830323
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA2638373.3694267784355767No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC1850762.3635806594440614No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT700890.895097175429417No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA690350.8816366834420495No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC660650.8437072136104731No Hit
AAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTGA538300.6874556771157461Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTG487810.622975578402066Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA465470.594445465404173Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGA368130.47013386293260184No Hit
AACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTGA281020.35888685562524053Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCT275870.3523098600147146Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAG273930.3498323121536621No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT248770.3177008151515589No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC241950.3089910850420857No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC233410.2980847661073496No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC172740.22060392655577554No Hit
AAGCAGGTCGTCTACGAATGGTCTAGGATCGTCGGACTGTAGAACTCTGA170450.21767939841051256Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCAGAGTACTAACCACTATACGATCACGGATCGTCGGACTGTAGAACTCT165680.21158769567998664Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAA158130.20194569240630303No Hit
GGGGAGAGGGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT150850.19264850249472468Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGGATCGTCGGACTGT146930.1876423233115671No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAAC146330.18687607139577767No Hit
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT144180.1841303353641989Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTGAA135730.17333895421683115Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTGAA129270.16508897525683167Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCCCGCCTCACCGGGTCAGTGAAAAAACGGATCGTCGGACTGTAGAACTC125500.16027435905262144Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTG118020.15072175183577996Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCCGATCGTCGGACTGTAGAACT110410.1410031233705174No Hit
AGCAGGTCGTCTACGAATGGTCTAGGATCGTCGGACTGTAGAACTCTGAA97700.12477135362104474Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATAGTCAAGTTCGACCGTCTTCTCAGCGATCGTCGGACTGTAGAACTCT89300.1140438267999928Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCACGGATCGTCGGACTGTAGAAC87740.11205157181894031No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAACT82180.10495097073262495No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGATCGTCGGA82130.10488711640630917No Hit
GGCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGA81490.10406978102946711Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCAGAGTACTAACCACTATACGATCACGGGATCGTCGGACTGTAGAACTC81180.10367388420630924Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTC79820.10193704653051988Illumina DpnII expression Sequencing Primer (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG1036650.042.967521
GTGCGAT260450.041.8437231
AAGCCTA51200.041.0479241
AGAGTAC106300.039.6390953
GAGTACT106200.039.1027534
AGCGACG70600.037.1637761
GCAGAGT111850.037.0677641
TGCCTCG28000.036.4280361
GGCCCGA287950.036.285979
GTACTAA118300.036.139516
TGCGATC307300.036.093822
ACCCGGG90800.036.0048371
TACTAAC119200.035.9033557
GCCCGAG289100.035.86857210
CGGCCCG296350.035.782968
ACTAACC114500.035.662088
AGTACTA118500.035.6560175
CGACGCT80500.035.524533
GCGACGC79100.035.5008742
CTATACG128550.035.48840716
Produced by FastQC (version 0.11.2)