FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext6_GCCAAT-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext6_GCCAAT-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595404
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1668492.529776796084061No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1342552.0355841734638243No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA867671.3155676286092557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC782731.1867809765709576No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT758021.1493154930312077No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGA714901.0839366322366302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT525590.7969034194114568No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC443400.6722863375768945No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG431270.6538947424600525No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC425750.6455252779056446Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG400000.6064829387252092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG377660.5726108665974063No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTG359730.5454252688690489Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAA323410.4903566180327998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT293390.4448400734814729No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT257530.3904688780247578No Hit
CCTTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC254760.3862689836740858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA180270.27332669840998364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGTCGCCGCGCTCTCTCTCTCCCCAGATCGGAAGAGCACACGTCTGA158460.24025821617599166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG145720.22094173457759375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCGTCGCCGCGCTCTCTCTCTCCCAGATCGGAAGAGCACACGTCTGA141980.21527111910051305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC135240.20505188158299326No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT133310.2021256014036441No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT115970.1758345660099063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT115850.17565262112828872No Hit
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG105160.1594443645908575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCT102580.15553254963607993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT94360.14306932524527685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTAGATCGGAAGAGCACACGTCTGAAC89290.13538215399693485Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC85950.13031802145857932No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCCAGATCGGAAGAGCACACGTCT84100.12751303786697524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC83180.12611812710790726No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA82890.1256784269773315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGTTGAACGCACGTAGATCGGAAGAGCACACGTCTGAAC81920.12420770585092286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC77180.11702088302702912No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC76850.11652053460258084No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC76500.11598986203119627No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCTG75960.11517111006391723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTCT72330.10966727739498597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT71590.10854528395834434No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC68540.1039208515505646No Hit
GTCGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT66290.1005093850202353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACC183150.043.5567131
GTTGGGG219000.043.488621
ACCATTC221600.042.783395
ATTCGTA218900.042.7162138
CTTGGGG100750.042.6117741
AACCATT207900.042.556874
CGTGGGG339750.042.539131
TAAACCA190700.042.481552
CGTAGAC221250.042.35900511
AAACCAT196650.042.3393823
TAGACGA221400.042.14506513
GACGACC215200.042.1244415
TGCTTAG159600.042.09235822
ACGACCT215000.041.90777216
CGACCTG214700.041.8023617
CTGCTTA160150.041.800221
AGACGAC219800.041.78341314
CTCGCCC275700.041.766511
GTAGACG220650.041.7337212
ACGCACG306900.041.69717818
Produced by FastQC (version 0.11.2)