FastQCFastQC Report
Wed 23 Nov 2016
G126-G127-M1M20-NEBNext6_GCCAAT-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext6_GCCAAT-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595404
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAA2068303.1359716554133756No Hit
GGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAAC1967652.983365385956645No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACT1072381.6259504345753497No Hit
AAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTGA611510.9271759546496318Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAAC607500.9210959631889115No Hit
GAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTG564690.856187126671846Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA536840.8139607520631034Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT299700.454407341839863Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCT280430.4251900262667761Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC242300.3673770401327955No Hit
AACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTCTGA218370.3310941983235599Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC204960.3107618578027972No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGATCGTCGGACTGTA171410.2598931013172203No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGGATCGTCGGACTGTAGAACTC140110.21243581136197268Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCTG138890.2105860383988608Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAAGCAGGTCGTCTACGAATGGTTTAGGATCGTCGGACTGTAGAACTCTG133470.2023681945791342Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGGGAGAGGGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTCT123720.18758517294770724Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGAGAGAGAGAGCGCGGCGACGGGTGATCGTCGGACTGTAGAACTCTG123150.1867209347600238Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAACT108390.16434171432106356No Hit
CCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAACTC101140.15334921105666915Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATGAGAGTAGTGGTATTTCACCGGCGGCCGATCGTCGGACTGTAGAACTC101010.15315210410158347Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAACTC95350.14457037051862176Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGAACGTGCGTTCAACGTGACGGGCGAGGATCGTCGGACTGTAGAACTC90640.13742903391513242Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGAGACGGGCCGGTGGTGCGCCCTCGGCGGGATCGTCGGACTGTAGAACT87530.13271362906654394No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAGATCGTCGGACTGTAG82290.12476870256924369No Hit
GCCTCGATCAGAAGGACTTGGGCCCCCCCACGATCGTCGGACTGTAGAAC78790.1194619768553981No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAACGATCGTCGGACTGTAGA76420.11586856544345123No Hit
ATGAGAGTAGTGGTATTTCACCGGCGGCGATCGTCGGACTGTAGAACTCT75210.11403395455380748Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCTCGATCAGAAGGACTTGGGCCCCCCACGGATCGTCGGACTGTAGAACT73580.11156253657850224No Hit
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT73340.1111986468152671No Hit
GGGGGAGAGAGAGAGCGCGGCGACGGGTAGATCGTCGGACTGTAGAACTC71650.1086362563991531Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAACTCTG71440.10831785285632238Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGA70610.10705940075846755Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCTCGATCAGAAGGACTTGGGCCCCCCCGATCGTCGGACTGTAGAACT67860.10288983055473176No Hit
CAGTCAGCCAGTTCACCATAGTTTTCACGGATCGTCGGACTGTAGAACTC67780.10276853396698671Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATAGTCAAGTTCGACCGTCTTCTCAGCGATCGTCGGACTGTAGAACTCT66270.10047906087329904Illumina DpnII expression Sequencing Primer (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTCG870700.042.7399561
AAGCCTA51750.041.5221
AGAGTAC32700.038.7681353
GTGCGAT83250.038.0553171
ATGAGAG43650.037.9795231
GAGTACT33650.037.1583024
TGCCTCG25400.036.751151
CTTAGTA7850.036.7133188
AGCGACG59900.036.6076051
CTACCGG8300.036.3192632
CGACGCT64500.036.203943
GCCCTTC141750.036.1575241
AACCTTA5200.035.9836245
GCGACGC64950.035.8427282
ACCGGGT35350.035.7251610
CCCTTCA145750.035.6738322
TCACCTT147400.035.541776
GCTACCG8500.035.4846421
CCCGCCT39200.035.4752582
ATTGCGC149650.035.3122614
Produced by FastQC (version 0.11.2)