FastQCFastQC Report
Wed 23 Nov 2016
G137-M7-Index-12_CTTGTA_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M7-Index-12_CTTGTA_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22819517
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTC48750.019.36210627
CCTACGT49450.018.91405325
CGTATCG1901.0913936E-1118.6875273
CTACGTT50750.018.47299426
CCGCCGA15300.017.96158624
CCCGCCG16800.016.73817823
ACGTTCT57850.016.46302428
CCCTACG58150.016.44913324
CGCCCGC18500.015.38604221
GACTACC61150.015.29666119
GTCCGCG36650.015.05232844
ACTACCC64450.014.73990220
ACCCTAC67500.014.32833123
GTCGCGG9550.014.27444643
CGCGTCG9500.014.12557940
CTCGGAT19200.013.9478331
TACCCTA68950.013.72433122
GCCCGCC20950.013.63211622
CTCGGGT22950.013.6307821
GCTTGAC72400.013.5558115
Produced by FastQC (version 0.11.2)