FastQCFastQC Report
Wed 23 Nov 2016
G137-M7-Index-12_CTTGTA_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M7-Index-12_CTTGTA_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22819517
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT16050.013.2825161
GGCGATC11100.012.0172922
TCACGAA30400.011.44068315
GGGCGAT11550.011.1556441
ATCACGA30600.011.14136914
CGCGTCA21200.010.93989851
GCGATCT14450.09.9932233
AGGGCGA40800.09.8802734
GATCACG34950.09.81392413
ACGAAGG35550.09.78642117
CACGAAG36800.09.63480516
AATCGCG15850.09.6072271
GGGCGAG42750.09.42965235
CAGGGCG42900.09.39752833
CGAAGGT37100.09.34076618
GCGAGGC43550.09.11112437
GCGACAT22050.09.0292147
CGAAAAT12700.08.8542441
GACGTCG11900.08.84276924
GAGATAC49750.08.802833
Produced by FastQC (version 0.11.2)