FastQCFastQC Report
Wed 23 Nov 2016
G139-M10-Index-5_ACAGTG_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M10-Index-5_ACAGTG_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19199437
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA820740.4274812849980965No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG705400.3674066067666463No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC399840.20825610667646138No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG398150.20737587253209558No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA326610.17011436324929735No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA309520.16121306057047402No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC280760.14623345465807147No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC271290.14130101835798622No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT259570.13519667269410035No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA258400.13458727982492402No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA224940.11715968546369354No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT223140.11622215797265306No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG211680.11025323294636191No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG199800.10406555150549468No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT195270.10170610731970943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT57850.027.5269971
CCCCCGT16400.024.47057316
CGACCCA17150.020.90253320
TCGCGAT3600.020.1631572
TGATCAA84200.019.545864
CGTCCCA18500.019.37721320
GCCGATT9000.018.51112718
CCTCCCG348000.018.39033144
CGCGATT2750.018.35642616
GATCAAA95150.018.1106995
CCCCGTC21050.017.86712817
CTCGCTA163050.017.8095991
ATATATC96750.017.62071812
ATCGGCG4450.017.51694722
ATCGACG3200.017.35855721
TTGATCA95600.017.0924913
CGGCCCA26400.016.82997320
GAGTCGC40600.016.6552166
GTTGATC101850.016.4608152
TCGCTAT184950.015.9773362
Produced by FastQC (version 0.11.2)