FastQCFastQC Report
Wed 23 Nov 2016
G139-M10-Index-5_ACAGTG_BC9WPLANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M10-Index-5_ACAGTG_BC9WPLANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19199437
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1053730.5488338017411656No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC733670.3821309968620434No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG589060.30681107992906254No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT435420.22678790008269514No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT423000.22031896039451573No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC402250.20951135181724337No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC397310.2069383597029434No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC397170.20686544089808467No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC338430.1762707937737966No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC334950.17445824062445164No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT329030.1713748168761407No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA309700.16130681331957805No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT300550.15654104857345555No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC291710.15193674689523448No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCC290120.15110859761148204No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC279200.14542093083250307No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC261470.1361862850457542No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG253370.13196741133607198No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC248500.12943087862420133No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC235970.12290464558934722No Hit
CTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC224860.11711801757520286No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAG224190.11676904900909334No Hit
CGCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCA216370.11269601290912853No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCA212500.11068032880339149No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT208870.10878964836312649No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG207280.10796149907937405No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT201180.10478432258195904No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT196360.10227383230039507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC168200.033.9468841
GGTGGCG209600.027.1224162
TGGCGCA212700.026.2299884
ACGCACG10650.025.9689856
GTGGCGC220250.025.633073
TCGCACG6750.023.2181976
CACGATC27950.022.8823071
TGACGCA12500.022.1228964
ACGATCA31350.020.1401862
CGATCAC33350.019.139673
GACGCAC15300.018.6778165
GCGCACG315250.017.6849616
CGCGGTG35550.017.6007631
CCGGCGG73850.016.7577671
GGCGCAC346050.016.4229095
CGGGTAC8750.016.0962091
CGCACGC380200.015.9779167
GTGACGC17600.015.973413
CGGTGAC21000.015.722391
GGTGACG19550.015.6767242
Produced by FastQC (version 0.11.2)