FastQCFastQC Report
Wed 23 Nov 2016
G139-M12-Index7_CAGATC_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M12-Index7_CAGATC_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10527111
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA394740.3749746725383631No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG365610.3473032629749986No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA206860.19650215524468204No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG202220.19209448822188727No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC201610.19151503199690778No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA197090.1872213563626336No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC167720.15932196402222792No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC159100.15113358261350146No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT130070.12355716587390406No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG128670.12222726634116426No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA127180.12081187326703405No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT117860.11195854209193766No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG114850.10909925809654708No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA113270.10759837148102645No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT111260.10568901572330719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT30800.027.2815781
CCCCCGT7800.025.87979516
TCGCGAT2950.024.5934662
CGACCCA8900.022.40159620
CTCGCGA3600.020.8052651
ATCGACG1351.14469E-820.5663621
GCGGAAT4950.019.8595681
TGATCAA45300.019.1621284
GATCAAA50900.018.1770485
CCTCCCG184250.018.0686644
CGCGATT1708.29823E-917.81138816
ATATATC53850.017.56576712
TTGATCA50700.017.53693
CTCGCTA84650.017.1431451
CGTCCCA10150.016.90770520
CCGGGCT31250.016.477771
CCCCGTC11400.016.38020717
CGGCGGT5250.016.34124216
CGATTGT4200.016.1557043
GTTGATC56150.016.0885732
Produced by FastQC (version 0.11.2)