FastQCFastQC Report
Wed 23 Nov 2016
G139-M13-Index-8_ACTTGA_BC9WPLANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M13-Index-8_ACTTGA_BC9WPLANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19154234
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC1046090.5461403468288004No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG951870.4969501782216924No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC756240.39481610175588333No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC725710.3788770670756137No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG575800.3006123867965694No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC529700.2765446010526968No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC471490.2461544533704663No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCC450030.23495066417169175No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT449550.23470006683639763No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT423150.22091721339522113No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC403800.210815008316177No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT395490.20647654194889759No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC384120.20054051756911812No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC380310.19855140122022108No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC345650.1804561853008583No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC333870.17430610903051513No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT312750.16327982627757393No Hit
CGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCA309630.16165094359816215No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC291080.1519664007446082No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA287290.1499877259513484No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAG272270.14214611766776997No Hit
CTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC267880.1398541962053925No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC266420.13909196264387289No Hit
CGCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCA252940.1320543541443631No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC249070.13003391312855422No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG239030.12479225219865227No Hit
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCC228960.1195349289352944No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG218960.11431415111666694No Hit
CGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCG216620.11309248910710812No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT214620.11204833354338263No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC211410.1103724638636032No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC207330.10824238651360321No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT201650.10527698471262281No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT193750.10115257023590712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC165250.031.6641371
GGTGGCG204450.025.4892352
TCGCACG7650.025.3027956
TGGCGCA201300.024.9861644
GTGGCGC209850.024.536913
ACGCACG9500.023.0436176
CGGGTAC11450.019.7550531
TACCGGC9800.019.7505285
CGCGGTG42350.018.4758261
ACCGGCG12300.017.4232236
CGGCGAG17300.017.3442231
TGACGCA11800.017.1826554
TGTCGCA11800.017.1826554
GACGCAC13400.017.0237775
GGTGACG14550.016.942292
GCGCACG298100.016.921336
GTGACGC12200.016.8066643
GGCCTTA56100.015.6477434
GTACCGG12850.015.420024
CCGCACG9300.015.36241156
Produced by FastQC (version 0.11.2)