FastQCFastQC Report
Wed 23 Nov 2016
G139-M8-Index-2_CGATGT_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M8-Index-2_CGATGT_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19558245
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG659760.33733088014798873No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA522130.26696158065306985No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG303370.1551110541871216No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC283500.1449516559384546No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC237450.12140659859818712No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA233810.11954549091700202No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA218110.11151818580859377No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC213470.10914578480840179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT16900.025.5414216
CGTTGAT41800.023.6400281
CGACCCA17850.019.94405620
CGTCCCA18900.019.90476220
CCTCCCG325450.018.74293544
CTCGCTA116900.018.2079561
TCGCGAT3900.018.0108412
TCGAGTG15650.017.2052442
GCCCGGG106400.016.9237481
TCGCTAT128700.016.9010812
CCCGATT10750.016.5545331
CTCGAGT18750.016.2350621
CCCCGTC25700.016.1097517
CGGCCCA25150.015.9621620
CCGTTGA16600.015.93977451
TCCTCCC386400.015.91764943
CGCGACC7450.015.71540451
TGATCAA64650.015.67791754
GGTTCCT391550.015.66005840
TGGTTCC390650.015.55555839
Produced by FastQC (version 0.11.2)