FastQCFastQC Report
Wed 23 Nov 2016
G139-M9-Index-4_TGACCA_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M9-Index-4_TGACCA_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292338
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG565950.30939183389241987No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA480670.2627712214808189No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG239740.13106033794039887No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC237330.1297428464311123No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA189740.10372648920001369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT40500.025.3694171
CCCCCGT14150.024.972616
TCGCGAT3550.019.7758882
CGACCCA15900.019.52866420
CCTCCCG269850.018.74098244
CTCGCTA93050.018.6884571
ATCGGCG4450.017.51654222
CGTCCCA16900.017.47687120
CCCGATT9300.017.1130311
TCGCTAT103250.016.885322
TGATCAA63850.016.3427944
TCGAGTG13500.015.77432252
CCGATTG7900.015.6997182
TCCTCCC327700.015.613029543
CGCTGGT324600.015.46670636
CTCGCGA5150.015.4515721
GTTCCTC336300.015.1387641
GGTTCCT336550.015.06478640
TGGTTCC338600.014.99229639
GTACGTA13750.014.9789361
Produced by FastQC (version 0.11.2)