FastQCFastQC Report
Wed 23 Nov 2016
G139-M9-Index-4_TGACCA_BC9WPLANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M9-Index-4_TGACCA_BC9WPLANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292338
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC565100.30892715846383334No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG538490.29438008416420036No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT315840.17266245572326513No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG309560.16922932432147272No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT270840.14806199185691846No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC264400.14454139213915684No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC258780.14146906754073757No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC242670.13266210147658544No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT239970.13118607364460466No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC235580.12878616172519883No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC207060.11319493440368311No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC191630.10475970868240025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC103850.027.6659151
TGGCGCA116000.023.833574
GGTGGCG128000.022.3357932
GTGGCGC128350.022.0056483
TCGCACG4750.017.4638986
CGTATGC10150.016.824721
GCGCACG167250.016.5603926
CGCACGC203950.014.80888757
CGCGGTG27150.014.7047781
ACGCACG8450.014.1800896
CCGGCGG52550.013.9648591
GGCGCAC205450.013.8727735
GCACGCC219950.013.7520938
GCCCTCT212800.013.64075853
TTCCCGT122550.012.53124744
CACGCCT244350.012.4669029
GGCCTTA42800.012.4346654
CGGCGTA13500.012.2875314
ACGCCTG251550.012.16993510
GACGCAC11050.012.0938745
Produced by FastQC (version 0.11.2)