FastQCFastQC Report
Wed 23 Nov 2016
G140-M10-Index-11_GGCTAC_BC9WPLANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M10-Index-11_GGCTAC_BC9WPLANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22938767
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT15050.013.0122311
ATCACGA27350.011.90392514
GGGCGAT10950.011.7775241
ATCGTCG5750.011.50624224
GGCGATC11450.011.0492922
TCACGAA28200.010.87914915
CGCGTCA20150.010.5484671
CGTCGGA13500.010.501677526
GATCACG33250.09.91512813
GCGACAT23650.09.9086137
CGAAGGT33700.09.60605718
CACGAAG33850.09.37568816
GCGATCT13400.09.2632093
ACGAAGG34500.09.26178717
AGGGCGA36350.09.14134834
CGACATC27550.09.0942978
GCGAGGC39400.09.01937737
CGATCTG17850.08.9576344
GGGCGAG40400.08.79464535
GGGAGAT73150.08.6191411
Produced by FastQC (version 0.11.2)